A successful strategy for the recovering of active P21, an insoluble recombinant protein of Trypanosoma cruzi

Structural studies of proteins normally require large quantities of pure material that can only be obtained through heterologous expression systems and recombinant technique. In these procedures, large amounts of expressed protein are often found in the insoluble fraction, making protein purificatio...

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Main Authors: Santos, Marlus Alves dos, Teixeira, Francesco Brugnera, Moreira, Heline Hellen Teixeira, Rodrigues, Adele Aud, Machado, Fabrício Castro, Clemente, Tatiana Mordente, Brigido, Paula Cristina, Silva, Rebecca Tavares e., Purcino, Cecílio, Gomes, Rafael Gonçalves Barbosa, Bahia, Diana, Mortara, Renato Arruda, Munte, Claudia Elisabeth, Horjales, Eduardo, da Silva, Claudio Vieira
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3941101/
https://ncbi.nlm.nih.gov/pubmed/24590372
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04259
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