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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

The investigation of the intracellular protein levels of bacterial species is of importance to understanding the pathogenic mechanisms of diseases caused by these organisms. Here we describe a procedure for protein extraction from Burkholderia species based on mechanical lysis using glass beads in t...

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Detalhes bibliográficos
Main Authors: Velapatiño, Billie, Zlosnik, James E. A., Hird, Trevor J., Speert, David P.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3940604/
https://ncbi.nlm.nih.gov/pubmed/24192802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50730
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