Quantitation of endogenously occupied and unoccupied binding sites for 1,25-dihydroxyvitamin D3 in rat intestine.

The quantitative reversible dissociation of the 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]-receptor complex by the mercurial reagent mersalyl was used to develop an assay for endogenously occupied and unoccupied 1,25-(OH)2D3 binding sites. Incubation of intestinal cytosol preparations in buffer contain...

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Bibliographic Details

Published in:	Proc Natl Acad Sci U S A
Main Authors:	Massaro, E R, Simpson, R U, DeLuca, H F
Format:	Artigo
Language:	Inglês
Published:	National Academy of Sciences 1983
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393863/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6302695/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.9.2549
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