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Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

The quantification of gene expression at the single cell level uncovers novel regulatory mechanisms obscured in measurements performed at the population level. Two methods based on microscopy and flow cytometry are presented to demonstrate how such data can be acquired. The expression of a fluoresce...

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Detalhes bibliográficos
Main Authors: Pelet, Serge, Aymoz, Delphine, Durandau, Eric
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3938322/
https://ncbi.nlm.nih.gov/pubmed/24121725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50637
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