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Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome

BACKGROUND: Shotgun metagenomics has become an important tool for investigating the ecology of microorganisms. Underlying these investigations is the assumption that metagenome sequence data accurately estimates the census of microbial populations. Multiple displacement amplification (MDA) of microb...

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Autori principali: Marine, Rachel, McCarren, Coleen, Vorrasane, Vansay, Nasko, Dan, Crowgey, Erin, Polson, Shawn W, Wommack, K Eric
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937105/
https://ncbi.nlm.nih.gov/pubmed/24475755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2049-2618-2-3
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