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Use of synthetic oligonucleotide probes complementary to genes for human HLA-DR alpha and beta as extension primers for the isolation of 5'-specific genomic clones.

We have synthesized 175-nucleotide-long probes for the DNA of human histocompatibility antigens HLA-DR alpha and beta by extending on poly(A)+ mRNA from B-cell lines with short synthetic deoxyribonucleotide primers complementary to the predicted nucleotide sequence of the NH2 terminus of both polype...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Das, H K, Biro, P A, Cohen, S N, Erlich, H A, von Gabain, A, Lawrance, S K, Lemaux, P G, McDevitt, H O, Peterlin, B M, Schulz, M F, Sood, A K, Weissman, S M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393635/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6403940/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.6.1531
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