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A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining

Immunohistochemistry on mouse tissue utilizing mouse monoclonal antibodies presents a challenge. Secondary antibodies directed against the mouse monoclonal primary antibody of interest will also detect endogenous mouse immunoglobulin in the tissue. This can lead to significant spurious staining. The...

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Detalhes bibliográficos
Main Authors: Goodpaster, Tracy, Randolph-Habecker, Julie
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3935443/
https://ncbi.nlm.nih.gov/pubmed/24152994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155413511620
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