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Optimization of ELISA Conditions to Quantify Colorectal Cancer Antigen-Antibody Complex Protein (GA733-FcK) Expressed in Transgenic Plant

The purpose of this study is to optimize ELISA conditions to quantify the colorectal cancer antigen GA733 linked to the Fc antibody fragment fused to KDEL, an ER retention motif (GA733-FcK) expressed in transgenic plant. Variable conditions of capture antibody, blocking buffer, and detection antibod...

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Bibliographic Details
Main Authors: Ahn, Junsik, Lee, Kyung Jin, Ko, Kisung
Format: Artigo
Language:Inglês
Published: Mary Ann Liebert, Inc. 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3935366/
https://ncbi.nlm.nih.gov/pubmed/24555929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/mab.2013.0072
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