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Extending single molecule fluorescence observation time by amplitude-modulated excitation
We present a hardware-based method that can improve single molecule fluorophore observation time by up to 1500% and super-localization by 47% for the experimental conditions used. The excitation was modulated using an acousto-optic modulator (AOM) synchronized to the data acquisition and inherent da...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3935333/ https://ncbi.nlm.nih.gov/pubmed/24587894 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/2050-6120/1/3/037001 |
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