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Yeast 2-microns plasmid DNA replication in vitro: purification of the CDC8 gene product by complementation assay.

Extracts of the yeast Saccharomyces cerevisiae support DNA replication on exogenous yeast 2-microns plasmid DNA templates. A crude extract from a S. cerevisiae cell division cycle mutant, cdc8-1, expressed the temperature-sensitive phenotype since it could be inactivated at 42 degrees C in vitro. Th...

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Bibliografiske detaljer
Main Authors: Arendes, J, Kim, K C, Sugino, A
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC393441/
https://ncbi.nlm.nih.gov/pubmed/6338494
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