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Molecular cloning of double-stranded RNA virus genomes.

Genome double-stranded RNAs isolated from purified human reovirus (serotype 3) and rotavirus (Wa strain) were modified at the 3' termini by addition of oligo(C) approximately 15 with T4 RNA ligase. These RNAs were transcribed into cDNA by oligo(dG)-primed reverse transcriptase and cloned after inser...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Imai, M, Richardson, M A, Ikegami, N, Shatkin, A J, Furuichi, Y
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393379/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6300836/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.2.373
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