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Construction of a Rhizobium japonicum gene bank and use in isolation of a hydrogen uptake gene

A gene bank of Rhizobium japonicum DNA was constructed by using the broad host range conjugative cosmid pLAFR1. Eighty-three percent of the clones in the bank contained cosmids with insert DNA averaging 22.6 kilobase pairs in length. A series of cosmids containing a hydrogen uptake (hup) gene was id...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Cantrell, Michael A., Haugland, Richard A., Evans, Harold J.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393335/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593268/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.1.181
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