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Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

A cDNA library of approximately equal to 9,000 members has been prepared from chicken smooth muscle mRNA by using the plasmid expression vector pUC8. Addition of Sal I and EcoRI linkers at different stages during the preparation of the cDNA resulted in a population of molecules, most of which had Ec...

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Détails bibliographiques
Auteurs principaux: Helfman, D M, Feramisco, J R, Fiddes, J C, Thomas, G P, Hughes, S H
Format: Artigo
Langue:Inglês
Publié: 1983
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC393303/
https://ncbi.nlm.nih.gov/pubmed/6185958
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