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Detection of viral sequences of low reiteration frequency by in situ hybridization.
The sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cDNA excess, by increasing the diffusion of the cDNA through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. Saturation of intracellular RNA with [3H]cD...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1978
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC393131/ https://ncbi.nlm.nih.gov/pubmed/216014 |
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