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Next Generation Sequencing-based Parallel Analysis of Melting Kinetics of 4096 Variants of a Bacterial Promoter

Promoter melting by bacterial RNA polymerase is a key step in transcription initiation. We used Next Generation Sequencing (NGS) based approach to analyze in parallel promoter melting of all 4096 sequence variants of the 6 bp -10 promoter element. We used NGS read count for each sequence of a promot...

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Autors principals: Heyduk, Ewa, Heyduk, Tomasz
Format: Artigo
Idioma:Inglês
Publicat: 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3931131/
https://ncbi.nlm.nih.gov/pubmed/24359527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi401277w
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