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FACS purification of Drosophila larval Neuroblasts for next generation sequencing

Elegant tools are available for the genetic analysis of neural stem cell lineages in Drosophila, but a methodology for purifying stem cells and their differentiated progeny for transcriptome analysis is currently missing. Previous attempts to overcome this problem either involved using RNA isolated...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Harzer, Heike, Berger, Christian, Conder, Ryan, Schmauss, Gerald, Knoblich, Juergen A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3930877/
https://ncbi.nlm.nih.gov/pubmed/23660757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.062
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