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Efficient engineering of a bacteriophage genome using the type I-E CRISPR-Cas system
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system has recently been used to engineer genomes of various organisms, but surprisingly, not those of bacteriophages (phages). Here we present a method to genetically engineer the Escherichia coli phage T...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3929423/ https://ncbi.nlm.nih.gov/pubmed/24457913 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.27766 |
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