Optimal Immobilization of β-Galactosidase onto κ-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications

β-Galactosidase (β-gal) was immobilized by covalent binding on novel κ-carrageenan gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. 2(2) full-factorial central composite experiment designs were employed to optimize the conditions for...

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Bibliographic Details
Main Authors: Elnashar, Magdy M., Awad, Ghada E., Hassan, Mohamed E., Mohy Eldin, Mohamed S., Haroun, Bakry M., El-Diwany, Ahmed I.
Format: Artigo
Language:Inglês
Published: Hindawi Publishing Corporation 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3929382/
https://ncbi.nlm.nih.gov/pubmed/24672334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/571682
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