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Evaluation of the effectiveness of semen storage and sperm purification methods for spermatozoa transcript profiling

Different semen storage and sperm purification methods may affect the integrity of isolated spermatozoal RNA. RNA-Seq was applied to determine whether semen storage methods (pelleted vs. liquefied) and somatic cell lysis buffer (SCLB) vs. PureSperm (PS) purification methods affect the quantity and q...

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Detalhes bibliográficos
Main Authors: Mao, Shihong, Goodrich, Robert J., Hauser, Russ, Schrader, Steven M., Chen, Zhen, Krawetz, Stephen A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3927401/
https://ncbi.nlm.nih.gov/pubmed/23869956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3109/19396368.2013.817626
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