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Reconstitution of cholera toxin-activated adenylate cyclase.

Reconstitution of adenylate cyclase activity responsive to stimulation by guanylyl-5'imidodiphosphate or NaF may be achieved by mixing dilute Lubrol 12A9-solubilized extracts of wild-type S49 membranes with membranes of an adenylate cyclase-deficient variant. Experiments using N-ethylmaleimide to in...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Johnson, G L, Kaslow, H R, Bourne, H R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1978
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392724/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/277913/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.7.3113
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