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Optimization of ERK Activity Biosensors for both Ratiometric and Lifetime FRET Measurements

Among biosensors, genetically-encoded FRET-based biosensors are widely used to localize and measure enzymatic activities. Kinases activities are of particular interest as their spatiotemporal regulation has become crucial for the deep understanding of cell fate decisions. This is especially the case...

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Detalhes bibliográficos
Main Authors: Vandame, Pauline, Spriet, Corentin, Riquet, Franck, Trinel, Dave, Cailliau-Maggio, Katia, Bodart, Jean-François
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3926607/
https://ncbi.nlm.nih.gov/pubmed/24434874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s140101140
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