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Optimization of ERK Activity Biosensors for both Ratiometric and Lifetime FRET Measurements

Among biosensors, genetically-encoded FRET-based biosensors are widely used to localize and measure enzymatic activities. Kinases activities are of particular interest as their spatiotemporal regulation has become crucial for the deep understanding of cell fate decisions. This is especially the case...

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Hlavní autoři: Vandame, Pauline, Spriet, Corentin, Riquet, Franck, Trinel, Dave, Cailliau-Maggio, Katia, Bodart, Jean-François
Médium: Artigo
Jazyk:Inglês
Vydáno: Molecular Diversity Preservation International (MDPI) 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3926607/
https://ncbi.nlm.nih.gov/pubmed/24434874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s140101140
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