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Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens
Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing qua...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Public Library of Science
2014
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3925228/ https://ncbi.nlm.nih.gov/pubmed/24551186 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0088886 |
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