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Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.

A method is described for excising cloned DNA segments that have been inserted into their vectors by poly(dA-dT) joins. The recombinant DNA is cleaved within the vector DNA portion by one or more restriction endonucleases to generate a linear DNA molecule with the insert DNA sequence flanked by the...

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Bibliografische gegevens
Hoofdauteurs: Goff, S P, Berg, P
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1978
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC392420/
https://ncbi.nlm.nih.gov/pubmed/347445
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