Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity.

Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein splicing elements (inteins) are present in many archaeal DNA polymerases, but only the DNA polymerase from strain GB-C contained an intein. Of t...

Descrición completa

Gardado en:
Detalles Bibliográficos
Main Authors: Southworth, M W, Kong, H, Kucera, R B, Ware, J, Jannasch, H W, Perler, F B
Formato: Artigo
Idioma:Inglês
Publicado: 1996
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC39236/
https://ncbi.nlm.nih.gov/pubmed/8643567
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!

Títulos similares