Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity.

Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein splicing elements (inteins) are present in many archaeal DNA polymerases, but only the DNA polymerase from strain GB-C contained an intein. Of t...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Southworth, M W, Kong, H, Kucera, R B, Ware, J, Jannasch, H W, Perler, F B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8643567/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.11.5281
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