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Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions

Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredi...

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Detalhes bibliográficos
Main Authors: Kubaczka, Caroline, Senner, Claire, Araúzo-Bravo, Marcos J., Sharma, Neha, Kuckenberg, Peter, Becker, Astrid, Zimmer, Andreas, Brüstle, Oliver, Peitz, Michael, Hemberger, Myriam, Schorle, Hubert
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3923226/
https://ncbi.nlm.nih.gov/pubmed/24527396
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2013.12.013
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