A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol

Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low. Furthermore, it is not suitable for cryopreservation of large numbers of hPSCs. While slow-freezing method...

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Hauptverfasser: Imaizumi, Keitaro, Nishishita, Naoki, Muramatsu, Marie, Yamamoto, Takako, Takenaka, Chiemi, Kawamata, Shin, Kobayashi, Kenichiro, Nishikawa, Shin-ichi, Akuta, Teruo
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3922972/
https://ncbi.nlm.nih.gov/pubmed/24533137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0088696
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