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Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins.

A procedure is described for immunizing in vitro and stimulating proliferation of specific B-cell lymphocytes. The method is applicable to production of monoclonal antibodies against proteins that are soluble only in denaturing solvents. An induction period is described in which antigen is presented...

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Detalhes bibliográficos
Main Authors: Van Ness, J, Laemmli, U K, Pettijohn, D E
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC392260/
https://ncbi.nlm.nih.gov/pubmed/6083563
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