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Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
The principle of a DNA cloning procedure that directionally generates genomic DNA fragments 50-2000 kilobases away from an initial probe is presented. The method depends on partial digestion of high molecular weight genomic DNA and subsequent ligation at very low concentration to generate covalent D...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1984
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392022/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6093122/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.21.6812 |
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