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Heat-labile alkaline phosphatase from Antarctic bacteria: Rapid 5′ end-labeling of nucleic acids

A heat-labile alkaline phosphatase has been purified to near homogeneity from HK47, a bacterial strain isolated from Antarctic seawater. The active form of the enzyme has a molecular weight of 68,000 and is uniquely monomeric. The optimal temperature for the enzymatic activity is 25°C. Complete and...

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Détails bibliographiques
Auteurs principaux: Kobori, Hiromi, Sullivan, Cornelius W., Shizuya, Hiroaki
Format: Artigo
Langue:Inglês
Publié: 1984
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC391996/
https://ncbi.nlm.nih.gov/pubmed/16593525
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