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Kinetic analysis of mutations affecting the cII activation site at the PRE promoter of bacteriophage lambda.

Abortive initiation and run-off transcription assays were used to study the effects of cy mutations on activation of the phage lambda PRE promoter by cII gene product. Six point mutations in the repeated T-T-G-C sequences that flank the -35 consensus region of PRE decreased the apparent affinity of...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Shih, M C, Gussin, G N
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1984
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391938/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6238332/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.20.6432
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