A Loop-Mediated Isothermal Amplification (LAMP) Assay for Strongyloides stercoralis in Stool That Uses a Visual Detection Method with SYTO-82 Fluorescent Dye

An assay to detect Strongyloides stercoralis in stool specimens was developed using the loop-mediated isothermal amplification (LAMP) method. Primers were based on the 28S ribosomal subunit gene. The reaction conditions were optimized and SYTO-82 fluorescent dye was used to allow real-time and visua...

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Main Authors: Watts, Matthew R., James, Gregory, Sultana, Yasmin, Ginn, Andrew N., Outhred, Alexander C., Kong, Fanrong, Verweij, Jaco J., Iredell, Jonathan R., Chen, Sharon C-A., Lee, Rogan
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society of Tropical Medicine and Hygiene 2014
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3919238/
https://ncbi.nlm.nih.gov/pubmed/24323513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.13-0583
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