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Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract.

A serum-free medium with bovine pituitary extract as the only undefined supplement has been developed for long-term culture of human mammary epithelial cells. This medium supports serial subculture of normal cells for 10-20 passages (1:10 splits) without conditioning or special substrates, and it su...

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Autors principals: Hammond, S L, Ham, R G, Stampfer, M R
Format: Artigo
Idioma:Inglês
Publicat: 1984
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC391719/
https://ncbi.nlm.nih.gov/pubmed/6591199
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