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Binding of [3H]forskolin to rat brain membranes.

[12-3H]Forskolin (27 Ci/mmol) has been used to study binding sites in rat brain tissue by using both centrifugation and filtration assays. The binding isotherm measured in the presence of 5 mM MgCl2 by using the centrifugation assay is described best by a two-site model: Kd1 = 15 nM, Bmax1 (maximal...

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Autores principales: Seamon, K B, Vaillancourt, R, Edwards, M, Daly, J W
Formato: Artigo
Lenguaje:Inglês
Publicado: 1984
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC391641/
https://ncbi.nlm.nih.gov/pubmed/6433343
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