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Binding of [3H]forskolin to rat brain membranes.

[12-3H]Forskolin (27 Ci/mmol) has been used to study binding sites in rat brain tissue by using both centrifugation and filtration assays. The binding isotherm measured in the presence of 5 mM MgCl2 by using the centrifugation assay is described best by a two-site model: Kd1 = 15 nM, Bmax1 (maximal...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Seamon, K B, Vaillancourt, R, Edwards, M, Daly, J W
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1984
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Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391641/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6433343/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.16.5081
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