Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.
Two temperature-sensitive mutations have been constructed in the single actin gene (ACT1) of the yeast Saccharomyces cerevisiae by in vitro mutagenesis of the cloned gene followed by integrative transformation of mutagenized DNA into yeast cells. A strategy of allele replacement was used that allowe...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1984
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391597/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6379652/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.15.4889 |
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