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Saturated excitation of fluorescent proteins for subdiffraction-limited imaging of living cells in three dimensions

We report, for the first time, the saturated excitation (SAX) of fluorescent proteins for subdiffraction-limited imaging of living cells in three-dimensions. To achieve saturation, a bright yellow and green fluorescent protein (Venus and EGFP) that exhibits a strong nonlinear fluorescence response t...

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Detalhes bibliográficos
Main Authors: Yamanaka, Masahito, Saito, Kenta, Smith, Nicholas I., Kawata, Satoshi, Nagai, Takeharu, Fujita, Katsumasa
Formato: Artigo
Idioma:Inglês
Publicado em: The Royal Society 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3915824/
https://ncbi.nlm.nih.gov/pubmed/24511385
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1098/rsfs.2013.0007
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