Human apolipoprotein E expression in Escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein E.

Human apolipoprotein E (apoE) was produced in Escherichia coli by transforming cells with an expression vector containing a reconstructed apoE cDNA, a lambda PL promoter regulated by the thermolabile cI repressor, and a ribosomal binding site derived from the lambda cII or the E. coli beta-lactamase...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Vogel, T, Weisgraber, K H, Zeevi, M I, Ben-Artzi, H, Levanon, A Z, Rall, S C, Innerarity, T L, Hui, D Y, Taylor, J M, Kanner, D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391503/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3909150/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.24.8696
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