Human apolipoprotein E expression in Escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein E.

Human apolipoprotein E (apoE) was produced in Escherichia coli by transforming cells with an expression vector containing a reconstructed apoE cDNA, a lambda PL promoter regulated by the thermolabile cI repressor, and a ribosomal binding site derived from the lambda cII or the E. coli beta-lactamase...

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Main Authors: Vogel, T, Weisgraber, K H, Zeevi, M I, Ben-Artzi, H, Levanon, A Z, Rall, S C, Innerarity, T L, Hui, D Y, Taylor, J M, Kanner, D
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC391503/
https://ncbi.nlm.nih.gov/pubmed/3909150
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