A PCR–High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

We have developed a PCR–high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput s...

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Bibliographische Detailangaben
Hauptverfasser: Pickering, Janessa, Binks, Michael J., Beissbarth, Jemima, Hare, Kim M., Kirkham, Lea-Ann S., Smith-Vaughan, Heidi
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2014
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911354/
https://ncbi.nlm.nih.gov/pubmed/24478508
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02191-13
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