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The EutT Enzyme of Salmonella enterica Is a Unique ATP:Cob(I)alamin Adenosyltransferase Metalloprotein That Requires Ferrous Ions for Maximal Activity
ATP:co(I)rrinoid adenosyltransferase (ACAT) enzymes convert vitamin B(12) to coenzyme B(12). EutT is the least understood ACAT. We report the purification of EutT to homogeneity and show that, in vitro, free dihydroflavins drive the adenosylation of cob(II)alamin bound to EutT. Results of chromatogr...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3911174/ https://ncbi.nlm.nih.gov/pubmed/24336938 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.01304-13 |
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