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The EutT Enzyme of Salmonella enterica Is a Unique ATP:Cob(I)alamin Adenosyltransferase Metalloprotein That Requires Ferrous Ions for Maximal Activity

ATP:co(I)rrinoid adenosyltransferase (ACAT) enzymes convert vitamin B(12) to coenzyme B(12). EutT is the least understood ACAT. We report the purification of EutT to homogeneity and show that, in vitro, free dihydroflavins drive the adenosylation of cob(II)alamin bound to EutT. Results of chromatogr...

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Detalhes bibliográficos
Main Authors: Moore, Theodore C., Mera, Paola E., Escalante-Semerena, Jorge C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911174/
https://ncbi.nlm.nih.gov/pubmed/24336938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.01304-13
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