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PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus

An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfull...

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Detalhes bibliográficos
Main Authors: Klevanskaa, Karina, Bier, Nadja, Stingl, Kerstin, Strauch, Eckhard, Hertwig, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911060/
https://ncbi.nlm.nih.gov/pubmed/24362421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03720-13
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