Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinica...

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Detalhes bibliográficos
Main Authors: Leal, C.A.G., Carvalho-Castro, G.A., Cottorello, A.C., Leite, R.C., Figueiredo, H.C.P.
Formato: Artigo
Idioma:Inglês
Publicado em: Brazilian Society of Microbiology 2013
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3910209/
https://ncbi.nlm.nih.gov/pubmed/24516428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-83822013005000054
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