Reconstitution of the GTP-dependent adenylate cyclase from products of the yeast CYR1 and RAS2 genes in Escherichia coli.

Plasmids carrying the CYR1 gene of yeast Saccharomyces cerevisiae, which encodes adenylate cyclase, were introduced into the cya mutant strain of Escherichia coli. The transformants had a GTP-independent adenylate cyclase activity but did not produce cAMP. The E. coli transformant carrying the yeast...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Uno, I, Mitsuzawa, H, Matsumoto, K, Tanaka, K, Oshima, T, Ishikawa, T
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1985
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390868/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2999779/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.7855
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