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Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli.

Human beta-globin was synthesized in Escherichia coli as a cleavable fusion protein, using the expression vector pLcIIFX beta-globin [Nagai, K. & Thøgersen, H. C. (1984) Nature (London) 309, 810-812]. The fusion protein cIIFX beta-globin was purified to homogeneity and cleaved at the junction by...

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Bibliografske podrobnosti
Main Authors: Nagai, K, Perutz, M F, Poyart, C
Format: Artigo
Jezik:Inglês
Izdano: 1985
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC390827/
https://ncbi.nlm.nih.gov/pubmed/3903751
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