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Human apolipoprotein B cDNA clone isolation and demonstration that liver apolipoprotein B mRNA is 22 kilobases in length.

An expression library made in plasmids pUC8 and pUC9 with mRNA derived from the human hepatoma cell line HepG2 was screened with a rabbit antiserum to human low density lipoprotein (LDL). Approximately 12,000 clones were screened and five positives were identified. The cDNA inserts were all 1500-160...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Huang, L S, Bock, S C, Feinstein, S I, Breslow, J L
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1985
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390780/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2995989/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.20.6825
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