Bacteriophage T7 DNA polymerase: cloning and high-level expression.
Phage T7 DNA polymerase consists of a 1:1 complex of the viral T7 gene 5 protein and the host cell thioredoxin. A 3.25-kilobase T7 DNA fragment containing the complete coding sequence of gene 5, and the nearby genes 4.7 and 5.3, was cloned in the BamHI site of the plasmid pBR322. Transformation of t...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1985
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390771/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2995984/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.20.6783 |
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