Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template

Quantitative real-time polymerase chain reaction (qRT-PCR) is a precise method to measure changes in gene transcript level. Accurate quantification requires careful RNA quality assessment, determination of primer efficiency, and selection of an appropriate reference gene. While many experimental pro...

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Hlavní autoři: Phillips, Michael A., D’Auria, John C., Luck, Katrin, Gershenzon, Jonathan
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer-Verlag 2009
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3906740/
https://ncbi.nlm.nih.gov/pubmed/24489433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11105-008-0072-1
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