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Expression of genes transferred into monocot and dicot plant cells by electroporation.

We have developed a general method for electrically introducing DNA into plant cells. Gene transfer occurs when a high-voltage electric pulse is applied to a solution containing protoplasts and DNA. Carrot protoplasts were used as a model system to optimize gene-transfer efficiency, which was measur...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Fromm, M, Taylor, L P, Walbot, V
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390645/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3862099/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.17.5824
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