Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator

A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase liquid chromatography. Based on automated gas-phase sequencing and subsequent identification of carboxyl-...

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Main Authors: Rao, K. Ranga, Riehm, John P., Zahnow, Cynthia A., Kleinholz, Lewis H., Tarr, George E., Johnson, Linda, Norton, Sheila, Landau, Matthew, Semmes, Oliver J., Sattelberg, R. Mark, Jorenby, William H., Hintz, Mary F.
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
Assuntos:
Biological Sciences: Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC390559/
https://ncbi.nlm.nih.gov/pubmed/16593589
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spelling pubmed-3905592004-04-23 Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator Rao, K. Ranga Riehm, John P. Zahnow, Cynthia A. Kleinholz, Lewis H. Tarr, George E. Johnson, Linda Norton, Sheila Landau, Matthew Semmes, Oliver J. Sattelberg, R. Mark Jorenby, William H. Hintz, Mary F. Proc Natl Acad Sci U S A Biological Sciences: Biochemistry A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase liquid chromatography. Based on automated gas-phase sequencing and subsequent identification of carboxyl-terminal amide, we have assigned the primary structure of this peptide as Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Asp-Ala-NH (2). We have confirmed the sequence by synthesizing this peptide and demonstrating that the synthetic PDH and the native PDH display identical chromatographic behavior and biological activity. This hormone is a member of a family of invertebrate neuropeptides that includes a light-adapting/pigment-dispersing octadecapeptide hormone from the prawn Pandalus borealis. In assays for melanophore pigment dispersion in destalked fiddler crabs, Uca PDH was 21-fold more potent than Pandalus PDH. These two hormones share a hexapeptide core sequence (residues 5-10: -Ile-Asn-Ser-Ile-Leu-Gly-) as well as the amino- and carboxyl-terminal residues but differ at positions 3, 4, 11, 13, 16, and 17. These results point to speciesrelated or group-specific structural differences among crustacean PDHs. 1985-08 /pmc/articles/PMC390559/ /pubmed/16593589 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Biological Sciences: Biochemistry
spellingShingle Biological Sciences: Biochemistry
Rao, K. Ranga
Riehm, John P.
Zahnow, Cynthia A.
Kleinholz, Lewis H.
Tarr, George E.
Johnson, Linda
Norton, Sheila
Landau, Matthew
Semmes, Oliver J.
Sattelberg, R. Mark
Jorenby, William H.
Hintz, Mary F.
Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
description A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase liquid chromatography. Based on automated gas-phase sequencing and subsequent identification of carboxyl-terminal amide, we have assigned the primary structure of this peptide as Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Asp-Ala-NH (2). We have confirmed the sequence by synthesizing this peptide and demonstrating that the synthetic PDH and the native PDH display identical chromatographic behavior and biological activity. This hormone is a member of a family of invertebrate neuropeptides that includes a light-adapting/pigment-dispersing octadecapeptide hormone from the prawn Pandalus borealis. In assays for melanophore pigment dispersion in destalked fiddler crabs, Uca PDH was 21-fold more potent than Pandalus PDH. These two hormones share a hexapeptide core sequence (residues 5-10: -Ile-Asn-Ser-Ile-Leu-Gly-) as well as the amino- and carboxyl-terminal residues but differ at positions 3, 4, 11, 13, 16, and 17. These results point to speciesrelated or group-specific structural differences among crustacean PDHs.
author Rao, K. Ranga
Riehm, John P.
Zahnow, Cynthia A.
Kleinholz, Lewis H.
Tarr, George E.
Johnson, Linda
Norton, Sheila
Landau, Matthew
Semmes, Oliver J.
Sattelberg, R. Mark
Jorenby, William H.
Hintz, Mary F.
author_facet Rao, K. Ranga
Riehm, John P.
Zahnow, Cynthia A.
Kleinholz, Lewis H.
Tarr, George E.
Johnson, Linda
Norton, Sheila
Landau, Matthew
Semmes, Oliver J.
Sattelberg, R. Mark
Jorenby, William H.
Hintz, Mary F.
author_sort Rao, K. Ranga
title Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
title_short Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
title_full Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
title_fullStr Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
title_full_unstemmed Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator
title_sort characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab uca pugilator
publishDate 1985
url https://ncbi.nlm.nih.gov/pmc/articles/PMC390559/
https://ncbi.nlm.nih.gov/pubmed/16593589
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