Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
We have constructed a series of plasmids containing a modified form of the phoA gene of Escherichia coli K-12 that have general utility for studies of protein secretion. In these plasmids, the promoter and signal sequence-encoding region of the phoA gene have been deleted; thus, expression of the ge...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390508/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3860846/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.15.5107 |
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