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False positive RNA binding activities after Ni-affinity purification from Escherichia coli
A His-tag is often added by means of recombinant DNA technology to a heterologous protein of interest, which is then over-produced in Escherchia coli and purified by one-step immobilized metal-affinity chromatography (IMAC). Owing to the presence of 24 histidines at the C-termini of the hexameric E....
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3904586/ https://ncbi.nlm.nih.gov/pubmed/23770724 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.25195 |
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