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Validation of the Isotropic Fractionator: Comparison with Unbiased Stereology and DNA Extraction for Quantification of Glial Cells

BACKGROUND: The “isotropic fractionator” (IF) is a novel cell counting technique that homogenizes fixed tissue, recovers cell nuclei in solution, and samples and quantifies nuclei by extrapolation. Studies using this technique indicate that the ratio of glia to neurons in the human brain is approxim...

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Detalhes bibliográficos
Main Authors: Bahney, Jami, von Bartheld, Christopher S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3904371/
https://ncbi.nlm.nih.gov/pubmed/24239779
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2013.11.002
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